DNA Transfection into a mouse (in-vivo)

Scientists performed in-vivo transfection in the fluids and matrixes. Plasmid DNA (PD) with linear polyethyleneimine can produce different complexes which are small and can quickly diffuse into the fluid. When this plasmid DNA is combined with 5 percent glucose, this produces homogenous complexes, which means the same kind of complexes, and their diameter range is 30 to 100nm, and the amount of PEI is 22kDa. Scientists observed that the complex formulated in the glucose showed a good diffusion rate in the CSF (cerebrospinal fluid). For this purpose, they used intraventricular injection. The diffusion rate was noticed in the newborn and adult mice. The brain ventricular are cavities used for communication. They diffuse from the one single site of injection and throughout the entire brain ventricular spaces. In the end, scientists determine the experiment’s efficacy using histochemistry, in which they observe the distribution of chemical components, like tissues, etc., of an enzyme’s beta-galactosidase activity. DNA transfection is found in neurons and in the glia, which are connective tissues of the brain. Thus this experiment proves the importance of in-vivo work and shows that the transfection technique can overcome many diseases and has a bright future.  

Another successful experiment was performed on the mouse. It was an in-vivo transfection experiment, and the researcher observed the stabilization of lipoplexes. 

DNA In-Vivo Transfection into Mouse Airways

Colloidal stability of lipid/DNA aggregates resulting from the aggregation is essential for lipid-based transfection, and stability is very difficult to get at a higher concentration of DNA. These aggregates are used for the in-vivo transfection. Researchers observed the potential of PEG conjugates for stabilizing the lipoplexes, and these lipoplexes are the result of the liposomes. In the experiment, researchers determined that PEG conjugates or derivatives can stabilize the lipoplexes formed at a high concentration of DNA. Further studies showed that PEG (stabilized) lipoplexes formed the DNA-coated structures or molecules and formed the clusters like aggregates resulting in complex morphologies. This method elaborates or shows the enhancing effect of the transfection of the mouse due to concentrated lipoplex solution through the intranasal process.